{"id":182,"date":"2016-03-02T11:02:05","date_gmt":"2016-03-02T11:02:05","guid":{"rendered":"http:\/\/carmenchifiriuc.ro\/?page_id=182"},"modified":"2017-11-30T23:56:24","modified_gmt":"2017-11-30T23:56:24","slug":"pn-ii-ru-te-2014-4-2037","status":"publish","type":"page","link":"http:\/\/carmenchifiriuc.ro\/?page_id=182","title":{"rendered":"PN-II-RU-TE- 2014-4-2037 \/ Contract no. 373"},"content":{"rendered":"

raport-de-faza-2015<\/a><\/p>\n

raport-de-faza-2016<\/a><\/p>\n

RAPORT DE ACTIVITATE FINAL<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Sura de finantare<\/strong><\/td>\nUNITATEA EXECUTIV\u0102 PENTRU FINAN\u0162AREA<\/p>\n

INV\u0102\u0162\u0102M\u00c2NTULUI SUPERIOR, A CERCET\u0102RII, DEZVOLT\u0102RII \u015eI INOV\u0102RII – TINERE ECHIPE DE CERCETARE\u00a0 – <\/strong>PN-II-RU-TE-2014-4<\/strong><\/td>\n<\/tr>\n

Titlul proiectului in engleza<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/td>\n

RISK ASSESSMENT OF TRANSPOSON-MEDIATED TRANSFER OF SOME CARBAPENEMASE GENES<\/td>\n<\/tr>\n
\u00a0Titlul proiectului in romana<\/strong><\/td>\nEVALUAREA RISCULUI DE TRANSFER AL UNOR GENE PENTRU CARBAPENEMAZE PRIN INTERMEDIUL TRANSPOZONILOR<\/p>\n

 <\/td>\n<\/tr>\n

Codul proiectului<\/strong><\/td>\nPN-II-RU-TE- 2014-4-2037<\/strong><\/td>\n<\/tr>\n
Numarul contractului<\/strong><\/td>\n373\/2015<\/strong><\/td>\n<\/tr>\n
Abstract (Romana)<\/strong><\/td>\nRezisten\u021ba bacililor Gram negativi (BGN) la carbapeneme reprezint\u0103 o problem\u0103 de s\u0103n\u0103tate public\u0103 la nivel global, provoc\u00e2nd infec\u021bii severe, dificil de tratat, cu rate mari de morbiditate \u0219i mortalitate.
\n\u00cen Rom\u00e2nia, a fost eviden\u021biat\u0103 in studii anterioare realizate de membrii echipei frecven\u021ba crescut\u0103 \u00een genofondul tulpinilor de BGN izolate din sec\u021biile de terapie intensiv\u0103 a genelor codificatoare de carbapenemaze (CPG) de tip NDM-1, OXA-48 \u0219i OXA-181 la enterobacterii, VIM-1 \u0219i VIM-2 la Pseudomonas aeruginosa, IPM-1, OXA-23, OXA-51 la Acinetobacter baumannii, \u00eens\u0103 bazele moleculare ale riscului de transfer sunt pu\u021bin investigate.
\nScopul acestui proiect este conceperea \u0219i dezvoltarea unor protocoale cu aportul biologiei moleculare, geneticii \u0219i bioinformaticii, pentru evaluarea riscului de transfer al unor CPG prezente la tulpini de Enterobacteriaceae, P. aeruginosa si A. baumannii, prin stabilirea poten\u021bialului de mobilizare prin transpozi\u021bie a acestor gene \u00een vederea identificarii clonelor cu risc crescut \u0219i izol\u0103rii focarelor critice.
\nAbordarea este inovatoare deoarece se bazeaz\u0103 pe testarea mediului genetic al rezisten\u021bei (secventa, nivelul de expresie si contextul genetic necesar mobilizarii), care va permite stabilirea unor criterii moleculare de apreciere a riscului de transfer al rezistentei la carbapeneme (gena\/alela CPG prezinta muta\u021bii ce pot cre\u0219te eficienta enzimei, se exprim\u0103 la un nivel ridicat, este \u00eencadrat\u0103 de elemente transpozabile active).<\/td>\n<\/tr>\n
Abstract (Engleza)<\/strong><\/td>\nGram negative bacilli (GNB) resistance to carbapenems represents a global public health issue, due to severe, hard to treat infections, with high rates of morbidity and mortality.
\nIn Romania, there was previously described by our research team the high prevalence of carbapenemase encoding genes (CRG) like NDM-1, OXA-48 and OXA-181 in enterobacterial strains, VIM-1, VIM-2 in Pseudomonas aeruginosa and IMP-1, OXA-23 and OXA-51 in Acinetobacter baumannii strains. The molecular mechanisms mediating the CRG transfer are not well investigated.
\nThe purpose of this project is designing and developing molecular protocols with the contribution of molecular biology, genetics and bioinformatics, for the assessment of some CRGs from Enterobacteriaceae, P. aeruginosa and A. baumannii transfer risk, by establishing the transposon-mediated mobilization potential of CRGs, that will make possible the identification of high-risk clones and isolation of critical outbreak sources.
\nThe approach of this project is innovative because it is based on testing the genetic environment of resistance (the sequence of CRG alleles, their molecular function and the required mobilization context), which will contribute to establish some molecular criteria for the evaluation or the CRGs risk level (the CRG gene\/allele have mutations that can increase the efficiency of the carbapenemase, the allele is overexpressed and it is framed by active transposable elements).<\/td>\n<\/tr>\n
Obiectivul proiectului<\/strong><\/td>\n <\/p>\n

Conceperea \u0219i dezvoltarea unor protocoale moleculare de evaluare a riscului de diseminare\/transfer a unor gene codificatoare pentru carbapenemaze prezente la tulpini de Enterobacteriaceae, P. aeruginosa, A. baumannii, in vederea identificarii clonelor cu risc crescut si izolarii focarelor critice. Prin obiectivele urmarite, se va urmari evaluarea potentialului de mobilizare a acestor gene prin intermediul fenomenului de transpozitie, care va contribui la \u00een\u021belegerea geneticii rezistentei la carbapeneme \u0219i a r\u0103sp\u00e2ndirii CPG \u00een mediul spitalicesc.<\/p>\n

 <\/td>\n<\/tr>\n

Rezultatele estimate<\/strong><\/td>\nPrin utilizarea de metode moleculare \u0219i bioinformatice vom dezvolta un protocol eficient la nivel de prototip, care va contribui la limitarea propagarii clonelor rezistente cu risc crescut in mediul spitalicesc, va permite investigarea riguroas\u0103 a mecanismelor care stau la baza rezisten\u021bei, cu impact direct asupra managementului fiec\u0103rui pacient, prin scaderea riscului de contaminare cu tulpini cu risc crescut, cu reducerea consecutiva a costurilor de \u00eengrijire si a ratelor de mortalitate, reducerea riscului de selectie a tulpinilor RA in microbiota individului si consecutiv, a diseminarii clonelor RA in comunitate, si prin furnizarea de informa\u021bii utile eforturilor de prevenire \u0219i de control al RA \u00een mediul spitalicesc.<\/td>\n<\/tr>\n
Echipa proiectului<\/strong><\/td>\nChifiriuc Mariana Carmen \u2013 Director Proiect<\/p>\n

Ecovoiu Alexandru – Responsabil stiintific<\/p>\n

Gheorghe Irina – Membru<\/p>\n

Czobor Ilda \u2013 Membru<\/p>\n

Gratiela Pircalabioru- membru<\/p>\n

Marcela Popa- membru<\/p>\n

Sarbu Ecaterina-Responsabil economic<\/td>\n<\/tr>\n

Buget<\/strong><\/td>\nCapitol de buget (cheltuieli)<\/strong><\/td>\nTotal\u00a0 buget 2015-2017<\/strong><\/p>\n

(Euro)<\/strong><\/td>\n<\/tr>\n

\u00a0<\/strong><\/td>\nPersonal<\/strong><\/td>\n75.000<\/strong><\/td>\n<\/tr>\n
\u00a0<\/strong><\/td>\nLogistic\u0103<\/strong><\/td>\n19.091<\/strong><\/td>\n<\/tr>\n
\u00a0<\/strong><\/td>\nDeplasare<\/strong><\/td>\n14.605<\/strong><\/td>\n<\/tr>\n
\u00a0<\/strong><\/td>\nIndirecte<\/strong><\/td>\n16.304<\/strong><\/td>\n<\/tr>\n
\u00a0<\/strong><\/td>\nTotal<\/strong><\/td>\n125.000<\/strong><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

raport-de-faza-2015 raport-de-faza-2016 RAPORT DE ACTIVITATE FINAL Sura de finantare UNITATEA EXECUTIV\u0102 PENTRU FINAN\u0162AREA INV\u0102\u0162\u0102M\u00c2NTULUI SUPERIOR, A CERCET\u0102RII, DEZVOLT\u0102RII \u015eI INOV\u0102RII – TINERE ECHIPE DE CERCETARE\u00a0 – PN-II-RU-TE-2014-4 Titlul proiectului in engleza \u00a0 \u00a0 RISK ASSESSMENT OF TRANSPOSON-MEDIATED TRANSFER OF SOME CARBAPENEMASE GENES \u00a0Titlul proiectului in romana EVALUAREA RISCULUI DE TRANSFER AL UNOR GENE PENTRU CARBAPENEMAZE … Continue reading PN-II-RU-TE- 2014-4-2037 \/ Contract no. 373<\/span> →<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-182","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/carmenchifiriuc.ro\/index.php?rest_route=\/wp\/v2\/pages\/182","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/carmenchifiriuc.ro\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/carmenchifiriuc.ro\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/carmenchifiriuc.ro\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/carmenchifiriuc.ro\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=182"}],"version-history":[{"count":11,"href":"http:\/\/carmenchifiriuc.ro\/index.php?rest_route=\/wp\/v2\/pages\/182\/revisions"}],"predecessor-version":[{"id":304,"href":"http:\/\/carmenchifiriuc.ro\/index.php?rest_route=\/wp\/v2\/pages\/182\/revisions\/304"}],"wp:attachment":[{"href":"http:\/\/carmenchifiriuc.ro\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=182"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}