Submissions to the GEO repository

Effects of Pseudomonas aeruginosa ATCC27853 ingestion on Drosophila melanogaster
Organism Drosophila melanogaster
Experiment type Expression profiling by array
Summary Drosophila melanogaster is a validated eukaryotic model for immunity-concerned studies in the post-genomic era. In the present study we performed oral experimental infection of D. melanogaster with Pseudomonas aeruginosa (strain ATCC27853). By using a whole genome microarray approach, we intended to identify significant alterations in the expression profile of relevant genes amenable to qualify as new models for the investigation of specific host-parasite interactions.
Overall design Wild type Oregon males and respectively γCOPS057302/TM6,Tb and γCOP14a/γCOP14a mutant males were feed with P. aeruginosa suspension (treated replicates) or with sucrose solution (untreated replicates). As a reference, we used Oregon untreated replicates (20 biological replicates). For the target groups we used four biological replicates and one target replicate per array.
Contributor(s) Ecovoiu AA, Ratiu AC, Fischer B, Barbu I, Chifiriuc CM
Submission date Apr 08, 2016
Last update date Apr 12, 2016

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