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Effects of Pseudomonas aeruginosa ATCC27853 ingestion on Drosophila melanogaster |
Organism |
Drosophila melanogaster |
Experiment type |
Expression profiling by array |
Summary |
Drosophila melanogaster is a validated eukaryotic model for immunity-concerned studies in the post-genomic era. In the present study we performed oral experimental infection of D. melanogaster with Pseudomonas aeruginosa (strain ATCC27853). By using a whole genome microarray approach, we intended to identify significant alterations in the expression profile of relevant genes amenable to qualify as new models for the investigation of specific host-parasite interactions. |
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Overall design |
Wild type Oregon males and respectively γCOPS057302/TM6,Tb and γCOP14a/γCOP14a mutant males were feed with P. aeruginosa suspension (treated replicates) or with sucrose solution (untreated replicates). As a reference, we used Oregon untreated replicates (20 biological replicates). For the target groups we used four biological replicates and one target replicate per array. |
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Contributor(s) |
Ecovoiu AA, Ratiu AC, Fischer B, Barbu I, Chifiriuc CM |
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Submission date |
Apr 08, 2016 |
Last update date |
Apr 12, 2016 |
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